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KMID : 0363420090230020029
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Journal of Korean Oriental Pediatrics
2009 Volume.23 No. 2 p.29 ~ p.50
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Anti-dermatitis Effects of KamiCheongsimyeonjatang on GATA-3 Regulation in NC/Nga Mouse
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Park Seul-Ki
Han Jae-Kyung
Kim Yun-Hee
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Abstract
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Objectives: The purpose of this study is to investigate effectiveness of KamiCheongsimyeonjatang(KCSYJT) medicines to suppress atopic dermatitis in mouse model experimentally.
Methods: First, in vitro, we isolated B cells from 18 weeks of atopicdermatitis-like skin NC/Nga mouse. Then we analyzed FACS(Fluorescence Activated Cell Sorter) by intracellular staining of IFN-γ, GATA-3+ analyzed cytokines by using real-time PCR. Secondly, in vivo, after administration of KCSYJT to atopic dermatitis NC/Nga mouse at 12 weeks of age, we analyzed serum IgE and the change of activated cell in PBMCs(Peripheral Blood Mononuclear Cells).
Results: In vitro, KCSYJT medicines supressed IL-1β, IL-6, TNF-α, and TGF- β mRNA and increased IL-10 mRNA in B cells. Also, KCSYJT medicines decreased the levels of GATA-3+CD4+ and increased the levels of IFN-γ+CD4+T Cell. In vivo, serum IgE levels dreased in KCSYJT group than control group and In PBMCs, the activated cell percentage of granulocytes, CD3+, CD3+/CD4+, B220+/CD23+, and CCR3+ decreased and CD19+, CD3+/CD8+ increased in KCSYJT group than control group.
Conclusions: This study demonstrates immunological activity of KCSYJT on atopic dermatitis-like model mice.
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KEYWORD
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Atopic dermatitis, KamiCheongsimyonjatang, NC/Nga mouse
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